PNC-28 20mg

Description

Strength: 20mg
CAS: 392661-17-5
Chemical Formula: C₁₆₄H₂₅₅N₄₇O₃₇S
Molecular weight: 3509.13 g/mol
Peptide Sequence: Glu-Thr-Phe-Ser-Asp-Leu-Trp-Lys-Leu-Leu-Lys-Lys-Trp-Lys-Met-Arg-Arg-Asn-Gln-Phe-Trp-Val-Lys-Val-Gln-Arg-Gly
Synonyms: PNC28, PNC 28, p53 17-26 penetratin peptide
Storage: Store 2–8 °C (≤–20 °C long-term). RT exposure during transport acceptable. Protect from light.
Shelf life: 24 months from the manufacturing date.

PNC-28 (20 mg) is a synthetic peptide derived from the amino acid sequence 17–26 of the human p53 protein, fused to a cell-penetrating sequence to facilitate intracellular delivery. It consists of 28 amino acids with the sequence:

Glu-Thr-Phe-Ser-Asp-Leu-Trp-Lys-Leu-Leu-Lys-Lys-Trp-Lys-Met-Arg-Arg-Asn-Gln-Phe-Trp-Val-Lys-Val-Gln-Arg-Gly

PNC-28 has been studied in experimental systems investigating p53-independent signaling and tumor cell biology. Research has focused on its interaction with membrane-expressed HDM-2, a key protein involved in cell membrane integrity and necrotic cell death pathways.

• Peptide sequence: Glu-Thr-Phe-Ser-Asp-Leu-Trp-Lys-Leu-Leu-Lys-Lys-Trp-Lys-Met-Arg-Arg-Asn-Gln-Phe-Trp-Val-Lys-Val-Gln-Arg-Gly
• Molecular weight:13 g/mol
• Molecular formula: C₁₆₄H₂₅₅N₄₇O₃₇S
• CAS number: 392661-17-5
• Purity: High research-grade purity (≥95% typical analytical standard)
• Supplied as 20 mg lyophilized peptide in sterile vial
• Derived from p53 (17–26) sequence with cell-penetrating domain
• Protect from light
• For laboratory research use only

PNC-28 is being researched for its potential interaction with cellular pathways associated with HDM-2, a key membrane-associated protein. In experimental systems, PNC-28 has been studied for its interaction with membrane-expressed HDM-2 in tumor cell lines, with experimental findings including ring-like pore assemblies and necrotic cell death in susceptible cancer cell systems. This mechanism distinguishes PNC-28 from other compounds studied for apoptosis.

Research applications have included:

• Cancer cell biology studies
• HDM-2 membrane expression studies
• p53-independent tumor cell membrane research
• In vitro tumor model systems

Preclinical investigations have examined how PNC-28 interacts with specific membrane proteins in malignant cell lines. Research remains experimental and limited to controlled laboratory environments.

Published studies involving PNC-28 have primarily been conducted in cell culture systems and animal models.

Reported experimental dosing has included:

• Microgram to milligram per kilogram (mg/kg) ranges in animal studies
• In vitro concentration-dependent exposure in tumor cell lines
• Short-term and repeated dosing protocols depending on study design

Observed findings in research contexts have included changes in cellular membrane characteristics and tumor cell viability markers in experimental models. These findings are limited to laboratory research and are not clinical recommendations. No approved therapeutic dosing guidelines exist.

• Store at 2–8 °C for standard handling
• For long-term storage, maintain at ≤–20 °C
• Shelf life is 24 months when stored under recommended conditions.
• Room temperature exposure during transport is acceptable
• Protect from light
• Avoid repeated freeze–thaw cycles
• Handle using appropriate laboratory protective equipment
• Dispose of materials in accordance with institutional chemical safety and biosafety guidelines

References

1. Bowne WB, Sookraj KA, Vishnevetsky M, et al. The penetratin sequence in the anticancer PNC-28 peptide causes tumor cell necrosis rather than apoptosis of human pancreatic cancer cells. Ann Surg Oncol. 2008;15(12):3588-3600. doi:10.1245/s10434-008-0147-0
2. Michl J, Scharf B, Schmidt A, et al. PNC-28, a p53-derived peptide that is cytotoxic to cancer cells, blocks pancreatic cancer cell growth in vivo. Int J Cancer. 2006;119(7):1577-1585. doi:10.1002/ijc.22029
3. Pincus MR, Silberstein M, Zohar N, Sarafraz-Yazdi E, Bowne WB. Poptosis or Peptide-Induced Transmembrane Pore Formation: A Novel Way to Kill Cancer Cells without Affecting Normal Cells. Biomedicines. 2024;12(6):1144. Published 2024 May 22. doi:10.3390/biomedicines12061144
4. National Center for Biotechnology Information. PubChem Compound Summary for CID 16158363, Pnc-28. https://pubchem.ncbi.nlm.nih.gov/compound/Pnc-28.

Compliance Notice

This product is intended for laboratory research use only and is not approved for human or veterinary use.